Structure of a small RNA hairpin

Small hairpin RNA as a small molecule sensor

Life has perfected the art of connecting the myriad molecular components in a live cell through regulatory links, transforming a cell from a mere protein shake into the most complex physical system known to man. Research in synthetic biology has strived, among other things, to recreate this complexity to a modest degree. This requires building new regulatory links and networks according to pre-...

RNA Isolation (Small RNA Extraction). Fatemeh Mehrpooyan*

Improved silencing vector co-expressing GFP and small hairpin RNA.

Small interfering RNA (siRNA) is a powerful tool for the specific silencing of gene expression. We developed an improved vector, pG-SUPER, that co-expresses green fluorescent protein (GFP) and small hairpin RNA simultaneously to facilitate analysis of silencing at the level of individual cells. As a test system, we analyzed lamin A/C knockdown in HeLa cells. The GFP signal was a reliable report...

Prediction of RNA Secondary Structure Including Kissing Hairpin Motifs

We present three heuristic strategies for folding RNA sequences into secondary structures including kissing hairpin motifs. The new idea is to construct a kissing hairpin motif from an overlay of two simple canonical pseudoknots. The difficulty is that the overlay does not satisfy Bellman’s Principle of Optimality, and the kissing hairpin cannot simply be built from optimal pseudoknots. Our str...

RNA editing in hepatitis delta virus genotype III requires a branched double-hairpin RNA structure.

RNA editing at the amber/W site plays a central role in the replication scheme of hepatitis delta virus (HDV), allowing the virus to produce two functionally distinct forms of the sole viral protein, hepatitis delta antigen (HDAg), from the same open reading frame. Editing is carried out by a cellular activity known as ADAR (adenosine deaminase), which acts on RNA substrates that are at least p...